Additional file 2 – Table 3: Information on primers used in pcr, amplified fragments and pcr conditions



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Additional file 2 – Table 3: Information on primers used in PCR, amplified fragments and PCR conditions

Gene region

Primer

Sequence 5´ - 3´

Reference

PCR program

18S rRNA

18A1

CCT ACT TCT GGT TGA TCC TGC CAG T

[60]

95°C 1min (95°C 30sec, 52,5°C 30sec, 72°C 30sec) x 30,

72°C 3min






1800

TAA TGA TCC TTC CGC AGG TT

[60]




18S long f

CTG GTT GAT YCT GCC AGT

[61]

94°C 2min, (94°C 1min, 50°C 1min, 72°C 1:30min) x 35,

72°C 3min.






18s long r

CTG ATC CTT CTG CCA GGT TC

[62]




18S intern f

CTG GTT GAT YCT GCC AGT

[61]

94°C 2min, (92°C 30s, 48°C 30s, 72°C 1min) x 40,

72°C 3min.






18S intern r

CTG GTG GTG CCC TTC CGT C

designed




18S short f

CTG GTT GAT YCT GCC AGT

[61]

94°C 2min, (92°C 30s, 48°C 30s, 72°C 40s) x 40,

72°C 3min.






18S short r

CTG AGA TCC AAC TAG GAG CTT

[62]

28S rRNA

28SC1

ACC CGC TGA ATT TAA GCA T

[17]

95°C 4min (94°C 30sec, 52°C 50sec, 72°C 2,5min) x 38,

72°C 10min






28SD3

GAC GAT CGA TTT GCA CGT CA

[17]

16S rRNA

16S-H

CGC CTG TTT ATC AAA AAC AT

[63]

according to 18S rDNA program (annealing temperature 52°C)




16S-R

CCG GTC TGA ACT CAG ATC ACG T

[63]




16s f

CGG CCG CCT GTT TAT CAA AAA CAT

[63]

92°C 2:30min, (90°c 30s, 46°C30s, 72°C 40s) x 10;

(90°C 30s, 50°C 40s , RAMP 0.3°C/s, 72°C 40s) x 30,



72° 3min




16s r

GGA GCT CCG GTT TGA ACT CAG ATC

[64]

COI

LCOI

GGT CAA CAA ATC ATA AAG ATA TTG G

[65]

according to 18S rDNA program (annealing temperature 52°C)




HCOI

TAA ACT TCA GGG TGA CCA AAA AAT CA

[65]




COI long f

GGT CAA CAA ATC ATA AAG ATA TTG G

[65] slightly modified

94°C 2:30min (90°C 30s, 48°C 1min, RAMP 0.3°C/sec, 72°C 1:30min) x 40.




COI long r

TAA AGA AAG AAC ATA ATG AAA ATG

[66]





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