2006: Master in Medical and Pharmaceutical Drug Innovation. Groningen University, Groningen, the Netherlands.
2003: B.Sc. in Biotechnology and Genetic Engineering. Jordan University of Science and Technology, Irbid, Jordan.
Significant achievements and Honors: Honor degrees in high school and B.Sc.
Employment History: Department of Biology and Biotechnology,Arab American University, Jenin, Palestine (2003-2004):
Duties: Coaching the students basic laboratorial techniques; such as molecular biology and microbiological techniques and mammalian and plant cell culturing.
Research experience: PhD project (till 2010): Propionic acid influence on human adipose tissue metabolism and inflammation.
It is well known that colonic-fermentation influences the development of obesity and its associated disorders. Therefore, we aimed to investigate the influence of propionic acid (PA), a metabolite produced by colonic-microbiota fermentation, on adipocytokines and chemokines production by human omental and subcutaneous adipose tissue. In addition, we aimed to investigate the underlying molecular mechanisms; the role of G-protein coupled receptors (GPCR), PPARγ and NF-kB, in PA signaling. Because obesity is associated with macrophage infiltration in adipose tissue, which is implicated in pathogenesis of inflammation associated disorders, we investigated the effect of PA on macrophages secretome. We found that PA influences the adipose tissue production of adipocytokines and chemokines. Furthermore, we found that PA exerts an immunoregulatory effects on macrophages. Regarding the underlying molecular mechanisms, we have shown that both PPARγ and GPCRs play a role in PA signaling; however NF-kB role still need to be investigated. These findings suggest that PA is involved in the regulation of human energy metabolism, inflammation and in turn obesity development and its associated disorders.
Master project (January-June 2005):Human scFvSIgA expressed on Lactococcus lactis as a vector for the treatment of mucosal disease.
The treatment of gastrointestinal tract pathologies, including cancer and inflammatory disease, remains problematic. Although substantial insight has been gained in the pathogenesis of these diseases, translating this knowledge to novel therapy remains troublesome. These problems are derived from the difficulty of delivering functional protein therapeutics to the mucosa of the digestive tract, mainly as a consequence of the protein-structure hostile nature of the lumen of the tract and the occurrence of unwanted side effects when such proteins are introduced in a systemic fashion. To overcome these troubles we have designed a novel recombinant scFvSIgA1 protein produced by Lactococcus lactis and anchored to its membrane to target colon cancer epitope, epithelial glycoprotein protein-2 (EGP-2). Accordingly L. lactis expressing this chimeric protein was capable of binding cells expressing this epitope. This proposes that the expression of specific antibodies on bacteria may allow local delivery of anticancer agents produced by such bacteria in conjunction with the antibody and provides a new avenue in the quest for targeted drug delivery.
Genetic Diversity Studies on Wheat Landraces in Palestine Using RAPD Markers in Comparison to Phenotypic Classification: United Nations Development Program, Department of Biology, Al-Quds University, Abu Dis, Palestine (4 months, 2003-2004).
This study was undertaken to examine the extent of genetic variation among the diverse individuals of important crops such as wheat. Moreover, we aimed to evaluate random amplification of polymorphic DNA (RAPD) as a molecular marker assay for genetic classification of cultivars of wheat and compare this approach with the authentic data collected from the same cultivars cultivated in Palestine. We found that RAPD technique could be used effectively as a valuable marker to discriminate between landraces and their original sources.
Al-maqased Hospital-Israel (2 months): learning molecular diagnosis of human genetic diseases, such as thalasemmia.
Alamani Center–Palestine (3 months): human in vitro fertelization (IVF) training.
First Medical Laboratories-Jordan (1 months): learning molecular and cytological technologies for genetic disorder diagnosis such as Hemophilia and Hepatitis a, b, c. Moreover, I was coached the basic histology and pathology techniques, such as tissue processing, embedding, microtomy and staining
Beleidsruimte 2006 grant, Graduate School for Drug Exploration), 1200 €.
SCFA for use in preventing, delaying and/or treating obesity and/or metabolic syndrome and/or insulin resistance and/or diabetes (P6019698EP).
Poster Presentations in Conferences:
Propionic Acid Stimulates Leptin Production by Human Adipose Tissue Through G-Protein Coupled Receptor(s). S. Al-Lahham, D. Weening, F. Rezaee, H. Roelofsen, K. Venema and R.J. Vonk. GUIDE early summer meeting, Groningen, the Netherlands, 2009.
Propionic Acid Stimulates Leptin Production In Human Adipose Tissue Through G-Protein Coupled Receptor(s). S. Al-Lahham, D. Weening, F. Rezaee, H. Roelofsen, K. Venema and R.J. Vonk. European Congress on Obesity, Amsterdam, the Netherlands, 2009.
Propionic Acid Stimulates Leptin Production In Human Adipose Tissue Through G-Protein Coupled Receptor(s). S. Al-Lahham, D. Weening, F. Rezaee, H. Roelofsen, K. Venema and R.J. Vonk. Keystone symposia Type 2 Diabetes and Insulin Resistance (J3) and Obesity: Novel Aspects of the Regulation of Body Weight (J4), Banff, AB, Canada, 2009.
The influence of short chain fatty acids on human adipose tissue function. S. Al-Lahham, H. Roelofsen, A. Hoek, K. Venema, R. J. Vonk
GUIDE early summer meeting, Groningen, the Netherlands, 2007.
Apoptosis induction to colon cancer cell by Recombinant BMP-2. S. Al-Lahham, S.Yuvaraj, W.Helfrich, L.F.M.H de Leij, M.P. Peppelenbosch, and N.A.Bos. GUIDE early summer meeting, Groningen, the Netherlands, 2005.
Oral presentations in Conferences:
Propionic Acid Stimulates Leptin Production In Human Adipose Tissue Through G-Protein Coupled Receptor(s). S. Al-Lahham, D. Weening, F. Rezaee, H. Roelofsen, K. Venema and R.J. Vonk. Annual Gut Day (Darmendag), Utrecht, the Netherlands, 2009.
S. Yuvaraj, S. Lahham, R. K. R. Marreddy, G. Dijkstra, W. A. M. Wolken, J. S. Lolkema, W. Helfrich, F-E. Johansen, M. P. Peppelenbosch, N. A. Bos. Human scFv SIgA expressed on Lactococcus lactis as a vector for the treatment of mucosal disease. Mol. Nutr. Food Res. 52, 913 – 920, 2008
Khaled Sawalha, Hatem Eideh, Sa’ad Laham, Haitham Hasesneh, Buthaina Mezeid. Genetic Diversity Studies on Wheat Landraces in Palestine Using RAPD Markers in Comparison to Phenotypic Classification.JABS, 2 (1): 29-34, 2008
Kees Meijer and Saed Lahham, Marcel de Vries, Marcel Bruinenberg, Desiree Weening, Martijn Dijkstra, Niels Kloosterhuis, Bert Blaauw, Nel Blom, J.J.L. van der Want, Roel Vonk and Fahrad Rezaee. Human Preadipocytes and Adipocytes Exhibit Immune Cell-Like Behavior. Submitted
Sa’ad H. Al-Lahham, Han Roelofsen,Desiree Weening, Martijn Dijkstra, Farhad Rezaee, Annemieke Hoek, Koen Venemaand Roel J. Vonk. Regulation of Adipokine Production in Human Adipose Tissue by Propionic Acid. Submitted
Sa’ad H. Al-Lahham, Han Roelofsen,Desiree Weening, Farhad Rezaee, Annemieke Hoek, Koen Venemaand Roel J. Vonk. Propionic Acid Influence on Human Adipose Tissue Inflammation and insulin resistance markers: the underlying molecular targets. Writing.
Sa’ad H. Al-Lahham, Desiree Weening, Han Roelofsen, Koen Venema and Roel J. Vonk. Propionic acid influence on THP-1 Macrophages Secretome; Quantitative Proteomics Approach. Writing.
Dr. J. Sikkema: Program Director, Top Institute Food and Nutrition, Wageningen, the Netherlands.
Dr. K. Venema: project manager at TNO, team leader at TNO quality of life and project leader at Top Institute Food and Nutrition, Ziest, the Netherlands.
Dr. F. Rezaee: Medical Biomics Centre, Department of Cell Biology, University Medical Centre of Groningen (UMCG), Groningen, the Netherlands.
Prof. Dr. N. A. Bos: Coordinator International Bachelor Medicine, Director Pre-University College, Institute for Medical Education, University Medical Centre of Groningen(UMCG), Groningen, the Netherlands.