Table S1 A. nidulans strains used in this study



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Table S1 A. nidulans strains used in this study


Strain

Genotype

Source

GR5

pyrG89 yA2; wA3; pyroA4

Lab stock

FGSC122

riboA1; nicB8

FGSC

R153

wA3; pyroA4

Lab stock

SO451

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

TNO2 x LO824

SO457

pyrG89 riboA1; nicB8

GR5 x FGSC122

SO459

pyrG89 riboA1 yA2; nicB8

GR5 x FGSC122

UI224

pyrG89 yA2; argB2

Eduardo Espeso

SWJ595

yA2 riboA1; argB2; nicB8

Steve James

LO1353

pyrG89 pabaA1; wA3; H1-mRFP::pyrGAf

Berl Oakley

HET-Δnup2-32

pyrG89; wA3; pyroA4; Δnup2::pyrGAf / pyrG89; wA3; pyroA4

Δnup2::pyrGAf heterokaryon in GR5

JD225

(pyrG89) 1; argB2; An-nup2-GFP::pyrGAf; nicB8; chaA1

SO451 GFP strain x SWJ595

SO570

pabaA1; An-nup2-GFP::pyrGAf; H1-mRFP::pyrGAf; nicB8; fwA1

JD225 x LO1353

ΔNup42-1

pyrG89; wA3; ΔAn-nup42::pyrGAf argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO739

yA2; ΔAn-nup42: ::pyrGAf

SO614 x SO459

SO614

pyrG89; wA3; argB2 ΔAn-nup42::pyrGAf

ΔNup42-1 x UI224

SO434

pyrG89; wA3; An-nup42-GFP::pyrGA.f.; pyroA4

Transformation in GR5

ΔNup49-C

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-nup49::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO634

pyrG89; wA3; argB2; ΔAn-nup49::pyrGAf ; fwA1 nirA14

ΔNup49-C x UI224

SO759

yA2; ΔAn-nup49::pyrGAf

SO634 x SO459

SO594

(pyrG89) 1; wA3; argB2; nicB8 An-nup49-GFP::pyrGAf ; fwA1 nirA14

SO451 GFP strain x SWJ595

ΔNup57-A

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-nup57::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO636

pyrG89; wA3; argB2; nirA14 ΔAn-nup57::pyrGAf

ΔNup57-A x UI224

SO761

yA2; ΔAn-nup57::pyrGAf

SO636 x SO459

SO596

(pyrG89) 1; wA3; argB2; sE15 An-nup57-GFP::pyrGAf

SO451 GFP strain x SWJ595

Het-Δnup82-B2

ΔAn-nup82::pyrGAf / pyrG89 An-nup82+

Δnup82::pyrGAf heterokaryon in SO451

SO597

(pyrG89)1 An-nup82-GFP::pyrGAf ; wA3; argB2;

SO451 GFP strain x SWJ595

ΔNup84-A

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-nup84::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO624

pyrG89 yA2; wA3; argB2; nirA14 ΔAn-nup84::pyrGAf

ΔNup84-A x UI224

SO749

yA2; ΔAn-nup84::pyrGAf

SO624 x SO459

SO605

pyrG89; argB2; pyroA4; chaA1 nirA14 An- nup84-GFP::pyrGAf

SO451 GFP strain x UI224

ΔNup85-A

pyrG89; wA3; ΔAn-nup85::pyrGAf argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO745

yA2; ΔAn-nup85::pyrGAf

ΔNup85-A x SO459

SO595

(pyrG89)1; wA3; argB2; An-nup85-GFP::pyrGAf

SO451 GFP strain x SWJ595

ΔNup120-A

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-nup120::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO622

pyrG89 yA2; wA3; argB2; pyroA4; fwA1 ChaA1 ΔAn-nup120::pyrGAf

ΔNup120-A x UI224

SO747

yA2; ΔAn-nup120::pyrGAf

SO622 x SO459

SO598

(pyrG89)1; wA3; argB2; fwA1 An-nup120-GFP::pyrGAf

SO451 GFP strain x SWJ595

ΔNup133-1

pyrG89; wA3; ΔAn-nup133::pyrGAf argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO630

pyrG89; argB2 ΔAn-nup133::pyrGAf ; nirA14 sE15 chaA1

ΔAn-nup133-1 x UI224

SO755

yA2; ΔAn-nup133::pyrGAf

SO630 x SO459

CDS234

pyrG89; wA3; An-Nup133-GFP::pyrGAf; pyroA4;

Gene replacement in GR5

Het-ΔNup159-12

ΔAn-nup159::pyrGAf / pyrG89 An-nup159+

Δnup159::pyrGAf heterokaryon in SO451

Het-ΔNup170-32

ΔAn-nup170::pyrGAf / pyrG89 An-nup170+

Δnup170::pyrGAf heterokaryon in SO451

Nup170-GFP

pyrG89 An-nup170-GFP::pyrGAf ; wA2; pyroA4

Eduardo Espeso

Het-ΔNup188-82

ΔAn-nup188::pyrGAf / pyrG89 An-nup188+

Δnup188::pyrGAf heterokaryon in SO451

SO599

(pyrG89)1 yA2; wA3; argB2; fwA1 An-nup188-GFP::pyrGAf

SO451 GFP strain x SWJ595

Het-ΔNup192-F2

ΔAn-nup192::pyrGAf / pyrG89 An-nup192+

Δnup192::pyrGAf heterokaryon in SO451

SO653

pyrG89; wA3; arB2; An-nup192-chRFP::pyrGAf

SO451 ChRFP strain x UI224

Het-ΔBrr6-32

ΔAn-brr6::pyrGAf / pyrG89 An-brr6+

Δbrr6::pyrGAf heterokaryon in SO451

Het-ΔCdc31-32

ΔAn-cdc31::pyrGAf / pyrG89 An-cdc31+

Δcdc31::pyrGAf heterokaryon in SO451

Het-ΔGle1-B2

ΔAn-gle1::pyrGAf / pyrG89 An-gle1+

Δgle1::pyrGAf heterokaryon in SO451

SO602

(pyrG89)1; wA3; argB2; sE15 An-gle1-GFP::pyrGAf

SO451 GFP strain x SWJ595

Het-ΔkapB-12

ΔkapB::pyrGAf / pyrG89 kapB+

ΔkapB::pyrGAf heterokaryon in SO451

ΔMlp1-C

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-mlp1::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO604

(pyrG89)1; wA3; argB2; An-mlp1-GFP::pyrGAf ; nicB8; fwA1 sE15

SO451 GFP strain x SWJ595

ΔNdc1-A

pyrG89; wA3; ΔAn-ndc1::pyrGAf argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO753

yA2; ΔAn-ndc1::pyrGAf

ΔNdc1-A x SO457

SO600

(pyrG89)1; wA3; argB2 An-ndc1-GFP::pyrGAf

SO451 GFP strain x SWJ595

Het-ΔNic96-12

ΔAn-nic96::pyrGAf / pyrG89 An-nic96+

Δnic96::pyrGAf heterokaryon in SO451

Nic96-GFP

pyrG89; wA3; pyroA4 An-nic96-GFP::pyrGAf

Eduardo Espeso

Het-ΔNsp1-52

ΔAn-nsp1::pyrGAf / pyrG89 An-nsp1+

Δnsp1::pyrGAf heterokaryon in SO451

ΔPom152-1

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-pom152::pyrGAf; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO741

yA2; ΔAn-pom152::pyrGAf

ΔPom152-1 x SO457

Het-Δran-12

ΔAn-ran::pyrGAf / pyrG89 An-ran+

Δran::pyrGAf heterokaryon in SO451

Het-ΔRanGAP-32

ΔAn-ranGAP::pyrGAf / pyrG89 An-ranGAP+

ΔranGAP::pyrGAf heterokaryon in SO451

Het-ΔRcc1-12

ΔAn-rcc1::pyrGAf / pyrG89 An-rcc1+

Δrcc1::pyrGAf heterokaryon in SO451

Rcc1-22

pyrG89 An-rcc1-GFP::pyrGAf; wA3; pyroA4;

Transformation in GR5

Het-ΔSac3-22

ΔAn-sac3::pyrGAf / pyrG89 An-sac3+

Δsac3::pyrGAf heterokaryon in SO451

SO555

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4 An-sac3-GFP::pyrGAf; se15 nirA14 chaA1 fwa1

GFP-tagged in SO451

Het-ΔSec13-12

ΔAn-sec13::pyrGAf / pyrG89 An-sec13+

Δsec13::pyrGAf heterokaryon in SO451

SO659

pyrG89; argB2 se12 An-sec13-ChRED::pyrGAf; pyroA4; fwA1

SO451 ChRED strain x UI224

ΔSeh1-A

pyrG89 ΔAn-seh1::pyrGAf ; wA3; argB2; ΔnkuA::argB pyroA4; se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO751

yA2; ΔAn-seh1::pyrGAf

ΔSeh1-A x SO459

Het-ΔsonA-12

ΔAn-sonA::pyrGAf / pyrG89 An-sonA+

ΔsonA::pyrGAf heterokaryon in SO451

ΔTrm-1

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; ΔAn-trm1::pyrGAf se15 nirA14 chaA1 fwa1

Gene deletion in SO451

SO618

pyrG89; argB2; pyroA4; chaA1 fwA1 ΔAn-trm1::pyrGAf

ΔTrm-1 x UI224

SO743

yA2; ΔAn-trm1::pyrGAf

SO618 x SO459

SO557

pyrG89; wA3; argB2; ΔnkuA::argB pyroA4; An-trm1-GFP::pyrGAf se15 nirA14 chaA1 fwa1

GFP-tagged in SO451

SO641

pyrG89; argB2; fwA1; pyroA4; sE15; An- trm1-GFP::pyrGAf

SO557 x UI224

1 In some strains we have not confirmed the pyrG89 marker which could be covered by the transformed pyrGAf.

2 For heterokaryon rescue strains in SO451 the complete genotype of both types of nuclei are not shown. The pertinent gene markers forcing the heterokaryotic state are indicated.




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